Counting chambers are mainly used for counting blood cells (e.g. leucocytes, erythrocytes, thrombocytes, …) and cells in fluid of the brain and spinal cord. Furthermore, counting chambers are also used to count bacteria, sperm and fungus spores.The hemocytometer (or haemocytometer) is a counting-chamber device originally designed and usually used for counting blood cells.Rulings cover 9 square millimeters. Boundary lines of Neubauer ruling are the center lines of the groups of three. The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10mm below the cover glass, so that the volume over each of the 16 small squares is. Counting pool Height 0.1mm 0.1mm Counting pool size 1mmx1mm 1mmx1mm Thickness 5mm 5mm Cell Depth 0.100mm±2% (1/10mm) 0.100mm±2% (1/10mm) Volume 0.1 µ…